Drug susceptibility testing of Mycobacterium tuberculosis by the broth microdilution method with 7H9 broth

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Drug susceptibility testing of Mycobacterium tuberculosis by the broth microdilution method with 7H9 broth.

In this study, we have evaluated the broth microdilution method (BMM) for susceptibility testing of Mycobacterium tuberculosis. A total of 43 clinical isolates of M. tuberculosis and H37Rv as a control strain were studied. All isolates were tested by the proportion method and the BMM for isoniazid (INH), rifampicin (RIF), streptomycin (STR), and ethambutol (ETM). The proportion method was carri...

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Standardization of broth microdilution method for Mycobacterium tuberculosis.

Indirect drug susceptibility tests of Mycobacterium tuberculosis was done to investigate the accuracy and feasibility of a broth microdilution method (BMM) for determining minimal inhibitory concentrations of conventional drugs against M. tuberculosis. Test drugs included isoniazid (H), rifampicin (R), ethambutol (E), streptomycin (S) and pyrazinamide (Z). Fifty isolates of M. tuberculosis from...

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The utility of Etest for antimicrobial susceptibility testing of Yersinia pestis was evaluated in comparison with broth microdilution and disk diffusion for eight agents. Four laboratories tested 26 diverse strains and found Etest to be reliable for testing antimicrobial agents used to treat Y. pestis, except for chloramphenicol and trimethoprim-sulfamethoxazole. Disk diffusion testing is not r...

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ژورنال

عنوان ژورنال: Memórias do Instituto Oswaldo Cruz

سال: 2004

ISSN: 0074-0276

DOI: 10.1590/s0074-02762004000100020